Long non-coding RNAs (lncRNAs) are molecules more than 200 nucleotides involved in several biological processes. Next Generation Sequencing allows to identify transcripts containing both coding and non-coding RNAs, but no strategies have been identified so far to discover ncRNA (non-coding RNA) biological functions; thus, most of the ncRNA functionalities are still unknown. We propose a new approach to detect putative lncRNAs transcripts starting from an RNA-seq analysis performed by a reference-based assembly. The extracted transcripts are then analyzed to filter out protein transcripts, detecting putative, thus interesting, lncRNAs submitted to biologists for further validations.
On the identification of long non-coding RNAs from RNA-seq
Tradigo, G.
2017-01-01
Abstract
Long non-coding RNAs (lncRNAs) are molecules more than 200 nucleotides involved in several biological processes. Next Generation Sequencing allows to identify transcripts containing both coding and non-coding RNAs, but no strategies have been identified so far to discover ncRNA (non-coding RNA) biological functions; thus, most of the ncRNA functionalities are still unknown. We propose a new approach to detect putative lncRNAs transcripts starting from an RNA-seq analysis performed by a reference-based assembly. The extracted transcripts are then analyzed to filter out protein transcripts, detecting putative, thus interesting, lncRNAs submitted to biologists for further validations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
